Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology

Molecular Characterization of Iraqi Local Goat Breeds Using Random Amplified Polymorphic DNA Markers

Cashmere is only produced from Maraz (Kurdi) goat breed in Iraqi Kurdistan Region. The objective of this study was to assess the genetic diversity of different Maraz color types, black goat and Shami breeds using random amplified polymorphic DNA (RAPD) markers. Fourteen primers were used and nine out of them were selected based on their number of bands (NB) and polymorphic characteristics. Thes...

Characterization of Pseudomonas aeruginosa in Burn Patients Using PCR- Restriction Frag-ment Length Polymorphism and Random Amplified Polymorphic DNA Analysis

One of the major opportunistic pathogens in patients with burn injuries is Pseudomonas aeruginosa, which causes severe infections in burned patients. The objective of the study was to examine the molecular epidemiology of P. aeruginosa colonization in the burn unit of Shahid Motahari Hospital in Tehran, Iran. Restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (...

Identification of Leishmania Species Causing Cutaneous Leishmaniasis Using Random Amplified Polymorphic DNA (RAPD-PCR) in Kharve, Iran

Background: Leishmaniasis, especially cutaneous leishmaniasis, is considered an important health problem in many parts of Iran including Kharve, Khorasan Razavi province. Cutaneous leishmaniasis is caused by various species of Leishmania, each having a different secondary host. Thus, identifying the parasites’ specie is of paramount importance for containment strategy planning. The morpho...

Molecular Identification of PCR and TaqMan Automated Detection in Utility of Random Amplified Polymorphic DNA

Random amplified polymorphic DNA (RAPD) fingerprinting of Mycoplasma gallisepticum isolates from chickens

  Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...